"Wood to Wheels" - Research Experience for High School Teachers in Sustainable Transportation Technologies


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Transformed bacteria showing the glowing phenotype (left) and non-transformed bacteria not glowing (right) in the pGlo lab .

RET Research poster by Jenn Coury and Robbie Olson (download larger image on right).












Bioengineering: DNA and Transformation

Teacher Infomation:Robbie Olsen

Hesperia High School, Biology


Unit OverviewStudents will be able to:

  • Describe the structure and function of DNA
  • Transform  bacteria and verify that the transformation was successful
  • Perform the most commonly used techniques for transformation
  • Explain gene expression

Target Grade Level: High School Biology

Download Unit Outline & Timeline

Download RET Research Poster

Lesson Plans:
Lesson One: What Are the Steps Necessary for DNA Extraction?

The purpose of this lab is for students to observe first hand that DNA is in the food that we eat, and to learn a simple method to extract DNA.  It is important to understand why each step is necessary due to the complex organization of DNA in cells and why it is important for Bioengineers and scientist to extract DNA from organisms

Download Lesson One Plans

Lesson Two:  How Do Scientists Manipulate DNA by Adding a Gene Through a Process Known as Transformation?

In this lesson/lab students will perform a procedure known as genetic transformation.  Using the Bio-rad Kit “pGLO” catalog number # 166-0003EDU. The teacher manual can be found at: http://www.biorad.com/webroot/web/pdf/lse/literature/ Bulletin_1660033EDU.pdf. Students will conduct an investigation on the bacterial transformation of a Green Fluorescent Protein gene taken from jellyfish.  Students will be expected to perform extremely specific laboratory techniques (pipetting, maintaining a sterile environment, and inoculation), record detailed observations, and analyze their findings.

Download Lesson Two Plans


Home || Lesson Plans  || Year 1 2011 Summary || Year 2 2012 Summary  || Year 3 2013 Summary